Real-time PCR CFX96 Dx

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CFX96 In Vitro Diagnostics (IVD) Real-Time PCR Systems combine the flexibility you need in your workflow and outstanding performance to deliver reliable results that your clinical laboratory demands.

Features Up to 5-target multiplexing for reduced sample and reagent use.
Quickly and accurately analyze and validate data using the intuitive CFX Manager Dx Software.
Reaction Volumes 1–50 µL
Supported Format 96-well plate
8-well strips

Thermal Cycler

Model C1000 Dx Thermal Cycler (not sold separately)
Maximum ramp rate 5°C/sec
Average ramp rate 3.3°C/sec
Heating and cooling method Peltier
Lid Heats up to 105°C


Range 0–100°C
Accuracy ±0.2°C of programmed target at 90°C
Uniformity ±0.4°C well-to-well within 10 sec of arrival at 90°C


Operational range 30–100°C
Programmable span 1–24°C
Module CFX96 Dx ORM
(Not sold separately)
Excitation 6 filtered LEDs
Detection 6 filtered photodiodes
Range of excitation/emission wavelengths 450–730 nm
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Calibrated dye FAM, SYBR™ Green, HEX, VIC, Quasar 670, Quasar 705, ROX, TEX 615, Texas Red, Cy5, Cy5.5, CAL Fluor Gold 540, CAL Fluor Orange 560, CAL Fluor Red 610

Scan Time

All channels 12 sec
FAM/SYBR Green only 3 sec



Operating systems

Windows 7, Windows 8, Windows 10
Multiplex analysis Up to 5 targets per well
Laboratory information
system (LIS) Compatible


Licensed for real-time PCR Yes
Sample capacity 12 x 8-tube strips
1 x 96-well plate (fast)
Sample size 10–50 µl (10–25 µl recommended)
Sample throughput 4 to 5 x 96-well plates per day
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H) 33 x 46 x 36 cm (12 x 18 x 14 in.)
Weight 21 kg (47 lb)